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ythdf3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc ythdf3
    Ythdf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf3/YTHDF3+Antibody/pm41862833-75-34-36
    Average 94 stars, based on 32 article reviews
    ythdf3 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Arsenic regulates ALKBH1 abundance and substrate specificity to promote translation and tumorigenicity
    Article Snippet: YTHDF2 , Proteintech , Cat# 24744–1-AP; RRID:AB_2687435. .. YTHDF3 , Cell Signaling Technologies , Cat# 24206S. .. Phospho-4E-BP1 (Ser65) , Cell Signaling Technologies , Cat# 9451S; RRID: AB_330947.

    Incubation:

    Article Title: N6-methyladenosine RNA landscape in the aged mouse hearts
    Article Snippet: .. Primary antibody incubation occurred at 4 °C overnight and secondary incubation occurred at room temperature for 1 h. Primary antibodies used were: anti p16 (1:500, Santa), anti p53 (1:1,000, CST), anti γH2A.X (1:1,000, Santa), anti METTL3 (1:1,000, Abcam), anti METTL14 (1:1,000, CST), anti WTAP (1:1,000, Proteintech), anti FTO (1:1,000, CST), anti ALKBH5 (1:1,000, Proteintech), anti YTHDC1 (1:1,000, CST), anti YTHDC2 (1:1,000, CST), anti YTHDF1 (1:1,000, CST), anti YTHDF2 (1:1,000, CST), anti YTHDF3 (1:1,000, CST) and anti GAPDH (1:10,000, Proteintech). .. Finally, SuperSignalTM ECL substrate (Thermo Scientific) was incubated with membranes for 2 min at room temperature and then exposed using the BioRad Chemidoc® imager.

    Article Title: Regulation and Function of the HPV16 CircE7 RNA
    Article Snippet: For western blot analysis, cells were lysed in Cell Lysis Buffer (Cell Signaling Technologies, 5872S), separated on SDS-PAGE gels, and transferred to PVDF membranes. .. Membranes were incubated overnight at 4°C with the following primary antibodies: YTHDC1 (Cell Signaling, 77422), YTHDF3 (Cell Signaling, 24206), YTHDC2 (Cell Signaling, 46324), FLAG (Sigma, A8592), GAPDH (Santa Cruz, sc-32233), HSP90 (Cell Signaling, 4877S). .. Western blots were then incubated with the appropriate HRP-conjugated secondary antibodies (Cell Signaling, 7076S and 7074S) and ECL substrate (Perkin Elmer, 50-904-9323) for signal detection.

    Article Title: miR-218a-5p derived from neural stem cell-exosomes inhibits ferroptosis in spinal cord injury through the Bmil/Mettl3/Alox12 axis.
    Article Snippet: Ferroptosis is a pivotal pathogenic mechanism in spinal cord injury (SCI) for which effective therapeutic strategies are lacking.. Neural stem cell-derived exosomes (NSC-exos) mitigate apoptosis and inflammation after SCI, but their role in ferroptosis remains unclear.. This study explored how NSC-exos regulate ferroptosis using rat SCI and H2O2-induced PC12 cell injury models.

    Lysis:

    Article Title: miR-218a-5p derived from neural stem cell-exosomes inhibits ferroptosis in spinal cord injury through the Bmil/Mettl3/Alox12 axis.
    Article Snippet: Ferroptosis is a pivotal pathogenic mechanism in spinal cord injury (SCI) for which effective therapeutic strategies are lacking.. Neural stem cell-derived exosomes (NSC-exos) mitigate apoptosis and inflammation after SCI, but their role in ferroptosis remains unclear.. This study explored how NSC-exos regulate ferroptosis using rat SCI and H2O2-induced PC12 cell injury models.

    Article Title: The m 6 A demethylase fat mass and obesity-associated protein mitigates pyroptosis and inflammation in doxorubicin-induced heart failure via the toll-like receptor 4/NF-κB pathway
    Article Snippet: The RIP assay was performed as per the manuals of the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Millipore, Billerica, MA, USA). .. In detail, the cells were submerged in the RIP lysis buffer and reacted with antibodies against FTO [1:50, #31687, Cell Signaling Technologies (CST), Beverly, MA, USA], YTH N6-methyladenosine RNA binding protein 1 (YTHDF1; 1:50, #57530, CST), YTHDF2 (1:100, #71283, CST), YTHDF3 (1:100, #24206, CST), or rabbit IgG (1:100, ab172730, Abcam) overnight at 4 °C, followed by washing with RIP washing buffer at 4 °C for 10 min and then with RIP lysis buffer at 4 °C for 5 min. ..

    Purification:

    Article Title: miR-218a-5p derived from neural stem cell-exosomes inhibits ferroptosis in spinal cord injury through the Bmil/Mettl3/Alox12 axis.
    Article Snippet: Ferroptosis is a pivotal pathogenic mechanism in spinal cord injury (SCI) for which effective therapeutic strategies are lacking.. Neural stem cell-derived exosomes (NSC-exos) mitigate apoptosis and inflammation after SCI, but their role in ferroptosis remains unclear.. This study explored how NSC-exos regulate ferroptosis using rat SCI and H2O2-induced PC12 cell injury models.

    Real-time Polymerase Chain Reaction:

    Article Title: miR-218a-5p derived from neural stem cell-exosomes inhibits ferroptosis in spinal cord injury through the Bmil/Mettl3/Alox12 axis.
    Article Snippet: Ferroptosis is a pivotal pathogenic mechanism in spinal cord injury (SCI) for which effective therapeutic strategies are lacking.. Neural stem cell-derived exosomes (NSC-exos) mitigate apoptosis and inflammation after SCI, but their role in ferroptosis remains unclear.. This study explored how NSC-exos regulate ferroptosis using rat SCI and H2O2-induced PC12 cell injury models.

    RNA Binding Assay:

    Article Title: The m 6 A demethylase fat mass and obesity-associated protein mitigates pyroptosis and inflammation in doxorubicin-induced heart failure via the toll-like receptor 4/NF-κB pathway
    Article Snippet: The RIP assay was performed as per the manuals of the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Millipore, Billerica, MA, USA). .. In detail, the cells were submerged in the RIP lysis buffer and reacted with antibodies against FTO [1:50, #31687, Cell Signaling Technologies (CST), Beverly, MA, USA], YTH N6-methyladenosine RNA binding protein 1 (YTHDF1; 1:50, #57530, CST), YTHDF2 (1:100, #71283, CST), YTHDF3 (1:100, #24206, CST), or rabbit IgG (1:100, ab172730, Abcam) overnight at 4 °C, followed by washing with RIP washing buffer at 4 °C for 10 min and then with RIP lysis buffer at 4 °C for 5 min. ..



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    <t>YTHDF3</t> is overexpressed in breast cancer tissues and cells. (A) YTHDF3 expression in breast cancer tissues and adjacent normal tissues in TCGA database; (B) YTHDF3 expression in different types of breast cancer in TCGA database; (C) Kaplan–Meier curves of OS in the TCGA dataset of all breast cancer patients with high and low YTHDF3 expression; (D) YTHDF3 expression in paired breast cancer tissues and adjacent normal tissues; (E) YTHDF3 expression of breast cancer and para‐cancerous tissues was evaluated by immunohistochemistry, ×100; (F) YTHDF3 protein expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453; (G) Relative YTHDF3 mRNA expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453.
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    YTHDF3 is overexpressed in breast cancer tissues and cells. (A) YTHDF3 expression in breast cancer tissues and adjacent normal tissues in TCGA database; (B) YTHDF3 expression in different types of breast cancer in TCGA database; (C) Kaplan–Meier curves of OS in the TCGA dataset of all breast cancer patients with high and low YTHDF3 expression; (D) YTHDF3 expression in paired breast cancer tissues and adjacent normal tissues; (E) YTHDF3 expression of breast cancer and para‐cancerous tissues was evaluated by immunohistochemistry, ×100; (F) YTHDF3 protein expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453; (G) Relative YTHDF3 mRNA expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: YTHDF3 is overexpressed in breast cancer tissues and cells. (A) YTHDF3 expression in breast cancer tissues and adjacent normal tissues in TCGA database; (B) YTHDF3 expression in different types of breast cancer in TCGA database; (C) Kaplan–Meier curves of OS in the TCGA dataset of all breast cancer patients with high and low YTHDF3 expression; (D) YTHDF3 expression in paired breast cancer tissues and adjacent normal tissues; (E) YTHDF3 expression of breast cancer and para‐cancerous tissues was evaluated by immunohistochemistry, ×100; (F) YTHDF3 protein expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453; (G) Relative YTHDF3 mRNA expression in normal human breast cell line MCF‐10A and breast cancer cell lines MCF‐7, MDA‐MB‐231, SK‐BR‐3, ZR‐75‐1 and MDA‐MB‐453.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Expressing, Immunohistochemistry

    Silence of YTHDF3 restrain cell proliferation and migration of breast cancer cells and arrested the cell cycle. (A) Relative expression of YTHDF3 mRNA and protein after transfection of shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells; (B) the growth ability of MDA‐MB‐231 and MCF‐7 cells transfected with shNC and shYTHDF3 was detected by CCK‐8 assay; (C) colony formation assay was used to detect the proliferation ability of MDA‐MB‐231 and MCF‐7 cells in shNC and shYTHDF3 groups; (D) the cell cycle distribution of MDA‐MB‐231 and MCF‐7 cells in shNC and shYTHDF3 groups was detected by flow cytometry; (E) expression of cyclin D1 and CDK4 in shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells; (F, G) transwell invasion assay was used to detect the migration ability of breast cancer cells in the shNC group and the shYTHDF3 group in MCF‐7 and MDA‐MB‐231 cells; (H) the expression of vimentin, MMP9, E‐cadherin, N‐cadherin and snail in shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: Silence of YTHDF3 restrain cell proliferation and migration of breast cancer cells and arrested the cell cycle. (A) Relative expression of YTHDF3 mRNA and protein after transfection of shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells; (B) the growth ability of MDA‐MB‐231 and MCF‐7 cells transfected with shNC and shYTHDF3 was detected by CCK‐8 assay; (C) colony formation assay was used to detect the proliferation ability of MDA‐MB‐231 and MCF‐7 cells in shNC and shYTHDF3 groups; (D) the cell cycle distribution of MDA‐MB‐231 and MCF‐7 cells in shNC and shYTHDF3 groups was detected by flow cytometry; (E) expression of cyclin D1 and CDK4 in shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells; (F, G) transwell invasion assay was used to detect the migration ability of breast cancer cells in the shNC group and the shYTHDF3 group in MCF‐7 and MDA‐MB‐231 cells; (H) the expression of vimentin, MMP9, E‐cadherin, N‐cadherin and snail in shNC and shYTHDF3 in MDA‐MB‐231 and MCF‐7 cells.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Migration, Expressing, Transfection, CCK-8 Assay, Colony Assay, Flow Cytometry, Transwell Invasion Assay

    Silencing YTHDF3 caused a decrease in lactate levels and affected glycolysis. (A) Lactate levels in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) extracellular acidification rate (ECAR) in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells measured by a seahorse instrument; (C) oxygen consumption rate (OCR) in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells measured by a seahorse instrument; (D) relative expression levels of hexokinase‐1 (HK1), hexokinase‐2 (HK2), PFKL, PKM, LDHA and LDHB in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (E) protein expression levels of PFKL, PKM, LDHA and LDHB in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; and (F) the correlation between YTHDF3 and PFKL, PKM, LDHA and LDHB was predicted using the GEPIA website.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: Silencing YTHDF3 caused a decrease in lactate levels and affected glycolysis. (A) Lactate levels in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) extracellular acidification rate (ECAR) in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells measured by a seahorse instrument; (C) oxygen consumption rate (OCR) in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells measured by a seahorse instrument; (D) relative expression levels of hexokinase‐1 (HK1), hexokinase‐2 (HK2), PFKL, PKM, LDHA and LDHB in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (E) protein expression levels of PFKL, PKM, LDHA and LDHB in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; and (F) the correlation between YTHDF3 and PFKL, PKM, LDHA and LDHB was predicted using the GEPIA website.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Expressing

    YTHDF3 regulates the glycolysis level of breast cancer through the mTOR–HIF1α–LDHA axis. (A) Relative mRNA expression of HIF1α in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) the correlation between YTHDF3 and HIF1α was predicted using the GEPIA website; (C) expression of key proteins in the mTOR–HIF1α–LDHA axis in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (D) after adding activator MHY1445, the growth ability of cancer cells in shNC and shYTHDF3 groups was detected by CCK‐8 assay; (E) the lactate level of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485; and (F) expression of proteins of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: YTHDF3 regulates the glycolysis level of breast cancer through the mTOR–HIF1α–LDHA axis. (A) Relative mRNA expression of HIF1α in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) the correlation between YTHDF3 and HIF1α was predicted using the GEPIA website; (C) expression of key proteins in the mTOR–HIF1α–LDHA axis in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (D) after adding activator MHY1445, the growth ability of cancer cells in shNC and shYTHDF3 groups was detected by CCK‐8 assay; (E) the lactate level of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485; and (F) expression of proteins of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Expressing, CCK-8 Assay

    YTHDF3 regulates breast cancer development through the mTOR–HIF1α–LDHA axis in vivo. (A) Tumour volumes in shNC and shYTHDF3 groups on the indicated days; (B) representative tumour images and (C) tumour weights in shNC and shYTHDF3 groups on day 63; (D) mRNA and (E) protein expression levels of YTHDF3, HIF1α and LDHA in the tumours in shNC and shYTHDF3 groups; (F) Immunohistochemical images of YTHDF3, Ki67, HIF1α and LDHA in tumours in the shNC and shYTHDF3 groups, ×100.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: YTHDF3 regulates breast cancer development through the mTOR–HIF1α–LDHA axis in vivo. (A) Tumour volumes in shNC and shYTHDF3 groups on the indicated days; (B) representative tumour images and (C) tumour weights in shNC and shYTHDF3 groups on day 63; (D) mRNA and (E) protein expression levels of YTHDF3, HIF1α and LDHA in the tumours in shNC and shYTHDF3 groups; (F) Immunohistochemical images of YTHDF3, Ki67, HIF1α and LDHA in tumours in the shNC and shYTHDF3 groups, ×100.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: In Vivo, Expressing, Immunohistochemical staining

    YTHDF3 is associated with prognosis in several cancers. (A) Column nomogram was constructed using YTHDF3 expression levels, age, T stage, N stage, M stage and tumour stage data of breast cancer patients; (B–E) survival analysis software package was used to analyse the survival prognosis difference between patients with high and low YTHDF3 expression in BRCA, DLBC, THCA and UVM.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

    doi: 10.1111/jcmm.71105

    Figure Lengend Snippet: YTHDF3 is associated with prognosis in several cancers. (A) Column nomogram was constructed using YTHDF3 expression levels, age, T stage, N stage, M stage and tumour stage data of breast cancer patients; (B–E) survival analysis software package was used to analyse the survival prognosis difference between patients with high and low YTHDF3 expression in BRCA, DLBC, THCA and UVM.

    Article Snippet: Primary antibodies used in the present study were listed as follows: GAPDH (ab313650, Abcam), HIF1 alpha (ab1, Abcam), mTOR (BM4182, Boster), p‐mTOR (BM4840, Boster), RICTOR (A03195‐1, Boster), RAPTOR (BM4438, Boster), AKT (BM4390, Boster), p‐AKT (BM4744, Boster), S6K (BM4240, Boster), p‐S6K (BM4141, Boster), LDHA (A1146, ABclonal), YTHDF3 (A8395, ABclonal), cyclin D1 (A11022, ABclonal), CDK4 (A23522, ABclonal), vimentin ( PAB48967 , Bioswamp), MMP9 (FNab05247, FineTest), LDHB (A5131, ABclonal), PKM1/2 (A27199PM, ABclonal), PFKL (A23687, ABclonal), snail (13099–1‐AP, Proteintech), E‐cadherin (PB9561, Boster), N‐cadherin (A01577‐3, Boster).

    Techniques: Construct, Expressing, Software